Are Blastocystis hominis and Cryptosporidium spp. playing a positive role in colorectal cancer risk? A systematic review and meta-analysis

Objective Intestinal protozoa Blastocystis hominis and Cryptosporidium spp. are two influential factors in intestinal complications and malignancies. In present study, we estimated the pooled prevalence and odds ratio (OR) of the two parasites in colorectal cancer (CRC) patients and their possible association with the deadly disease. Method Our systematic search was conducted for published researches between January 1, 2000 and April 30, 2022 by using four international databases include Scopus, PubMed, and Web of Science as well as Google scholar search engine. The random- and fixed-effects models were used to estimate the pooled prevalence, OR, and 95% confidence interval (CI) by comprehensive meta-analysis (V2.2, Bio stat) software. Inclusion and exclusion criteria were applied. Results Thirteen papers (seven case–control and six cross-sectional studies) for B. hominis/CRC and six papers (two case–control and four cross-sectional studies) for Cryptosporidium spp./CRC were eligible to include in data synthesis. Pooled prevalence of B. hominis and Cryptosporidium spp. in CRC patients was calculated to be 26.8% (95% CI 19.4–35.7%) and 12.7% (95% CI 6.8–22.5%), respectively. Based on case–control studies, significant difference was found between case and controls in both protozoa (B. hominis OR 2.10; 95% CI 1.39–3.18% vs. Cryptosporidium spp. OR 5.06; 95% CI 1.8–13.6%). Considering the Blastocystis subtypes, ST1 (5/6; 83.33% studies) and ST3 (5/6; 83.33% studies) had the highest number of reports in CRC patients. Regarding the Cryptosporidium species, only C. parvum and C. hominis were reported. Conclusion Given the significant prevalence of both parasites in CRC patients and their statistically significant association, there is a need to pay more attention to these two intestinal parasites in under treatment patients.


Introduction
According to the latest figures from the World Health Organization (WHO), 12 million cancerous case and 4 million deaths from cancer have been reported worldwide, of these, colorectal cancer (CRC) rank third in terms of morbidity (~ 2 million) and second in mortality rate (~ 1 million deaths) [1]. Chronic infections and inflammation along with unhealthy diet, stressful lifestyle, cell damages, constant exposure to radiation and harmful chemicals are risk factors for development of cancers [2,3]. Infectious agents including parasites account for approximately 16% of cancers [4]. As infectious factors, the Blastocystis hominis and Cryptosporidium spp. are ubiquitous opportunistic protozoa isolated from the human host gastrointestinal tract [5,6]. These prevalent enteric parasites may cause serious challenges in people undergoing colorectal cancer (CRC) chemotherapy (immunocompromised) due to their location in gastrointestinal tract [4,7]. Among the Cryptosporidium species, Cryptosporidium parvum (C. parvum) and Cryptosporidium hominis (C. hominis) are responsible for over 90% of all human cases [8]. So far, out of 22 identified B. hominis subtypes (ST1-ST22), ten subtypes have been isolated from humans (ST1-9 and ST12), which ST3 is more prevalent [9]. Both parasites have zoonotic potential and transmission routes are oral-fecal alongside the contaminated water and food sources as well as close animal contact [10,11]. Although the pathogenesis of B. hominis and Cryptosporidium spp. have not been clearly established, they have been frequently reported in individuals with gastrointestinal complications including diarrhea, abdominal cramps, etc. [12,13]. B. hominis and Cryptosporidium spp. has occasionally been controversially found in healthy people as well as people with gastrointestinal symptoms, the risk of being opportunistic in people undergoing chemotherapy cannot be ignored [14,15]. Up to now, numerous studies have been conducted on the pathogenic power and possible association of both protozoa with non-communicable diseases such as irritable bowel syndrome (IBS), Crohn's disease, and gastrointestinal cancers [16][17][18][19]. In the latter, scattered studies have shed new light on the potential virulence role and prevalence of parasites in CRC. The present systematic review and meta-analysis was designed and performed with the aim of aggregating the available data and providing a comprehensive and statistically documented picture of the pooled prevalence and odds ratio (OR) of B. hominis and Cryptosporidium spp. in CRC patients and their possible association with the deadly disease.

Search strategy
We followed the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines for the design, analysis and interpretation of the present study [20]. To evaluate the prevalence and OR of B. hominis and Cryptosporidium spp. in the CRC patients, a search was performed on the related literatures in four international databases, including Scopus, PubMed, and Web of Science as well as Google Scholar scientific search engine between January 1, 2000 and April 30, 2022. The searching process was accomplished using combinations of the following search keywords, including: "Blastocystis", "Cryptosporidium" AND "Colorectal cancer", "Gastrointestibal cancer" in English language.

Inclusion criteria
The following inclusion criteria were applied in the current review: (1) the peer-reviewed original research papers and short reports; (2) case-control and cross-sectional studies that estimated the prevalence of B. hominis and Cryptosporidium spp. in CRC patients; and (3) studies published with full text or abstracts in English which published online up to April 30, 2022.

Exclusion criteria
The exclusion criteria were included: (1) All types of review studies, editorials, letters and case reports; (2) those articles that were not available in English language; and (3) researches that report the prevalence of both parasites in cancers other than CRC, as well as studies with confusing and/or unclear data.

Study selection and data extraction
The primary screening of eligible studies based on inclusion criteria in mentioned databases was the responsibility of two expert researchers (AT and SB). Additionally, the references of the eligible papers were carefully handchecked to find relevant articles that were not retrieved in the database searching. After removing duplicate and irrelevant records, and ensuring the existence of extractable data, studies information was extract by the ER and AB. Extracted items included first author name, year of publication, study design, geographical location of study, total CRC patients sample size and the number of isolated B. homins and Cryptosporidium spp. Finally, the extracted data was double checked by AA and the controversial issues were resolved by AT.

Quality assessment
To assess the quality of included case-control studies, we used the Newcastle-Ottawa Scale (NOS), as suggested by the Cochrane collaboration [21]. In this 9-star scale, a study could be awarded a maximum of one star for each numbered item within the selection and exposure categories. Also, a maximum of two stars could be given for comparability. Papers with a total score of 0-3, 4-6 or 7-9 points were categorized as poor, moderate or of high quality, respectively. The Joanna Briggs Institute (JBI) checklist also was used for quality assessment of the included cross-sectional records which have contains ten questions with four options including, yes, no, unclear, and not applicable [22]. The papers with a total score of 4-6 and 7-10 points were classified as the moderate and high quality, respectively. We have decided to include (4-10 points) and exclude (≤ 3 points) the researches.

Data synthesis and statistical analysis
Data was analyzed using comprehensive meta-analysis software version 2. To assessment the association between B. homins and Cryptosporidium spp. with CRC, an OR and pooled prevalence using random-and fixed-effects models and corresponding 95% confidence intervals (CI) were calculated for each study. In order to assess heterogeneity of studies, I 2 value was considered that the value > 50% indicates a statistical significant heterogeneity. Eggers regression (Qualitative method) was applied to assess the possibility of publication bias during the analysis. P-value < 0.05 considered statistically significant.

Study characteristics
As shown in Fig. 1, a total of 1292 studies were identified by the initial search in the major databases. Finally, after removing duplicate and papers with non-related subjects, thirteen papers (seven case-control [4,[23][24][25][26][27][28] and six cross-sectional studies [29][30][31][32][33][34]) for B. hominis/CRC and six papers (two case-control [6,8] and four crosssectional studies [35][36][37][38]) for Cryptosporidium spp./CRC were eligible to include in data synthesis. These studies were conducted in ten different countries from four in Poland (one study for B. hominis and three studies for Cryptosporidium spp.), three in Iran (only B. hominis), two in China (one study for B. hominis and one study for Cryptosporidium spp.), two in Iraq (only B. hominis), two in Malaysia (only B. hominis), two in Saudi Arabia (only  Tables 1 and 2. The included articles in the present meta-analysis showed an acceptable quality.

Publication bias
Considering the B. hominis and CRC studies, detecting publication bias using the Eggers regression revealed that publication bias in case-control studies was not statistically significant (p value < 0.05). Due to the fact that only two case-control studies were performed on Cryptosporidium spp. and CRC, publication bias is not applicable.

Discussion
Over the past decade, there has been considerable evidence of parasitic infections, such as Cryptosporidium spp. and B. hominis, with various types of cancer [39,40]. Cryptosporidium spp. and B. hominis have been suggested as important intestinal parasites in CRC patients and the severe form of these diseases occurs most frequently in such patients [8,41]. In this regard, the current study is a systematic review and meta-analysis to address the pooled prevalence and ORs of Cryptosporidium spp. and B. hominis infections in CRC patients compared with non-cancer individuals. This meta-analysis revealed a positive association between Cryptosporidium spp. and B. hominis infections with CRC. Among the seven case-control studies regarding the B. hominis, five reported a significant and two reported a non-significant difference related with B. hominis infection in the case group compared to the control group (Table 1). Both case control studies on Cryptosporidium spp./CRC showed a significant prevalence of this protozoan in the case group compared to the control group (Table 2). High heterogeneity (I 2 ) was observed in this meta-analysis. Several sources of heterogeneity have been reported in the literature which include study design, detection method, geographical distribution, sample size, and high or low prevalence in some studies (different weights of each study) [42,43].
Considering B. hominis, some studies conducted on in vitro and in vivo, and epidemiological studies from human populations have revealed an association between B. hominis and CRC [27,41]. In this regard, in vitro studies have shown severe cytopathic and immunological effects by the solubilized antigen of B. hominis in human colorectal cancer cell line [41]. The findings of these studies suggest that B. hominis infection may increase the proliferative, invasive, and metastatic properties of CRC cells [41]. Another in vitro study indicated that the five subtypes of B. hominis significantly increased the proliferation of human CRC cell line HCT116, particularly ST3 [44]. The present systematic review has shown that ST1 and ST3 is more common in CRC patients than other subtypes. It is suggested that in the future studies, in order to deeper understanding the mechanism of these subtypes in CRC development, further cellular studies should be performed focusing on these subtypes in CRC patients.
Regarding the Cryptosporidium spp., several experimental and epidemiological studies have shown the potential role of cryptosporidiosis and CRC progression [19,38,45]. It has been suggested that C. parvum is one of the pathogen agents that may trigger intestinal dysplasia [45]. However, the pathophysiological mechanisms of Cryptosporidium spp. infection are multifactorial and not completely specified. An experimental study revealed that C. parvum is able to modulate host-cell cytoskeleton and intracellular signals, which may explain the transformed phenotype of the infected epithelial cells [46]. Moreover, our findings showed that several C. parvum  (IIa and IIc) and C. hominis (Ia) subtypes were present in CRC patients (Table 2). Therefore, it is suggested to evaluate the progression of CRC in laboratory and human studies by considering these species/subtypes. Some limitations of this systematic review and metaanalysis, which may affect the results, are listed as follows: (1) lack of access to the full text of some articles, (2) low sample size of some studies, (3) geographical dispersion of studies, (4) different diagnostic methods and (5) lack of some variables such as age and gender.
Also, the state of immunosuppression of the patients and the time of the evolution of the CRC may be another source of heterogeneity which was not considered in the present study.
In conclusion, the present meta-analysis demonstrates that CRC may be related with elevated risks of Cryptosporidium spp. and B. hominis infections. However, further studies should be performed to investigate the impact of Cryptosporidium spp. and B. hominis infections in the onset or development of CRC in the future.